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The out-of-distribution (OOD) generalization challenge is a longstanding problem in graph learning. Through studying the fundamental cause of data distribution shift, i.e., the changes of environments, significant progress has been achieved in addressing this issue. However, we observe that existing works still fail to effectively address complex environment shifts. Existing practices place excessive attention on extracting causal subgraphs, inevitably treating spurious subgraphs as environment variables. While spurious subgraphs are controlled by environments, the space of environment changes encompass more than the scale of spurious subgraphs. Therefore, existing efforts have a limited inference space for environments, leading to failure under severe environment changes. To tackle this issue, we propose a negative inference graph OOD framework (NeGo) to broaden the inference space for environment factors. Inspired by the successful practice of prompt learning in capturing underlying semantics and causal associations in large language models, we design a negative prompt environment inference to extract underlying environment information. We further introduce the environment-enhanced invariant subgraph learning to effectively exploit inferred environment embedding, ensuring the robust extraction of causal subgraph in the environment shifts. Lastly, we conduct a comprehensive evaluation of NeGo on real-world datasets and synthetic datasets across domains. NeGo outperforms baselines on nearly all datasets, which verify the effectiveness of our framework.