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Speech intelligibility can be substantially improved when speech and interfering noise are spatially separated. This spatial unmasking is commonly attributed to effects of head shadow and binaural auditory processing. In reverberant rooms spatial unmasking is generally reduced. In this study spatial unmasking is systematically measured in reverberant conditions for several configurations of binaural, diotic and monaural speech signals. The data are compared to predictions of a recently developed binaural speech intelligibility model. The high prediction accuracy (R2 > 0.97) indicates that the model is applicable in real rooms and may serve as a tool in room acoustical design.