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With the advancement of audio generation, generative models can produce highly realistic audios. However, the proliferation of deepfake general audio can pose negative consequences. Therefore, we propose a new task, deepfake general audio detection, which aims to identify whether audio content is manipulated and to locate deepfake regions. Leveraging an automated manipulation pipeline, a dataset named FakeSound for deepfake general audio detection is proposed, and samples can be viewed on website https://FakeSoundData.github.io. The average binary accuracy of humans on all test sets is consistently below 0.6, which indicates the difficulty humans face in discerning deepfake audio and affirms the efficacy of the FakeSound dataset. A deepfake detection model utilizing a general audio pre-trained model is proposed as a benchmark system. Experimental results demonstrate that the performance of the proposed model surpasses the state-of-the-art in deepfake speech detection and human testers.