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The current study investigates the effect of noise floor in measured room impulse responses (RIR) on the reproducibility of speech perception under spherical harmonics-based spatial sound reproduction. Subjective listening test measuring the intelligibility of speech in noise was conducted under the spatial sound reproduction implemented using practically measured RIR with varying level of noise floor. The same test was also conducted in the real rooms where the RIR were measured. The comparison of the experimental results from the spatial sound reproduction and the real room suggests using measured RIR with low noise floor contributes to reproducing speech perception in real rooms accurately when the room is highly reverberant. It also has an effect to improve the reproducibility when the sound sources are located at 5 m but not at 2 m. Truncating RIR to further remove the noise floor mostly did not help improve the reproducibility regardless of the acoustics of the room.